Calculators, trackers, and optimizers built by bioprocess engineers. Replace your scattered Excel sheets with purpose-built tools that work on your phone at the bench. Plus vendor-neutral side-by-side comparisons and independent sensor reviews.
Every tool a bioprocess scientist needs — from bench to bioreactor. Built for daily use, designed for mobile.
Convert RPM to RCF and back instantly. Rotor presets for Eppendorf, Beckman, Thermo Fisher centrifuges. Common protocol quick-reference. Mobile-friendly for bench use.
Paste amino acid sequence → get molecular weight, extinction coefficient (ε280), A280 for 1 mg/mL, estimated pI, charge vs pH curve. Common protein presets.
Calculate mass from molarity, percent solutions (w/v, v/v, w/w), concentration unit converter (M to mM, uM, nM). Built-in library of 50+ reagent molecular weights.
PCR, qPCR, RT-PCR reaction setup for N reactions with dead volume. 6 protocol presets (Standard PCR, SYBR, TaqMan, High-Fidelity, Colony PCR, RT). CSV export and pipetting guide.
Convert optical density to cells/mL, CFU/mL, and dry cell weight. Organism presets for E. coli, yeast, Pichia, Bacillus. Dilution factor correction and linearity warnings.
DNA and reagent volumes for PEI (N:P ratio), Lipofectamine 2000/3000, FuGENE HD, CaPO₄. Co-transfection with AAV triple and lentiviral 3rd-gen presets. Scale from 96-well to 50 L bioreactor.
Calculate primer melting temperature using nearest-neighbor (SantaLucia 1998), salt-adjusted, and basic methods. Primer pair analysis with quality scorecard, hairpin detection, and adjustable Na+, Mg2+, dNTPs.
A260 to concentration (ng/uL), mass-to-moles conversion (ng/uL to nM/uM for oligos, plasmids, RNA), Nanodrop purity check with 260/280 and 260/230 interpretation.
Stock solution prep, C1V1=C2V2 dilution, Henderson-Hasselbalch pH buffers, serial dilution planner, and common bioprocess buffer recipes (PBS, Tris, HEPES).
Hemocytometer cell counter with visual grid input. VCD, viability %, passage planner with vessel and organism presets. Multi-count growth tracking with charts.
Log VCD, viability, glucose, lactate, titer, pH, DO. Auto-calculates specific growth rate (µ), doubling time, qP, qGlc, IVC. Beautiful charts. CSV export. Works offline.
Compare bioreactor runs, build golden batch envelopes (mean ± sigma), align batches on biological events, find which variable caused a deviation via z-score contribution. Free SIMCA alternative.
Upload CSV from any bioreactor system. Auto-generate time-series charts for DO, pH, VCD, metabolites. Multi-run overlay. Derived metrics (µ, qP, IVC). Data never leaves your browser.
Paste OD or cell count data, auto-fit exponential, logistic, and Gompertz models. Extract µmax, doubling time, lag time. Model comparison with R² and AIC.
4-parameter logistic curve fitting for ELISA data. Standard curve with LLOQ/ULOQ, back-calculated sample concentrations, %CV flagging, accuracy validation.
BCA assay, Bradford assay, and direct A280 protein quantification. Standard curve fitting, Beer-Lambert law, dilution correction, and purification yield tracker.
MVD calculation, endotoxin limit by route (IV, intrathecal, ophthalmic), dilution series generator with pipetting volumes, PPC spike recovery validation. USP <85> compliant.
Calculate F₀ lethality for autoclave, SIP, and depyrogenation cycles. Moist heat (F₀), dry heat (FH), and depyrogenation (FD) modes with pass/fail validation and interactive charts.
Estimate media osmolality from component concentrations. Optimal range checker by cell type. Feed impact predictor. Conductivity-to-osmolality correlation.
Interactive decision tree for diagnosing common CHO problems. Viability dropping? Low titer? High lactate? Walk through systematic diagnosis with corrective actions.
Plan your complete seed train from vial to production bioreactor. Auto-selects vessels, calculates timing, media volumes, and generates a visual expansion diagram.
Calculate suspension volume, media volume, split ratio, and total cells needed for any target seeding density. Dual mode: suspension (cells/mL) and adherent (cells/cm²). 15 vessel presets.
Compare basal media costs, calculate supplement concentrations, estimate media cost per liter and per batch. Serum vs serum-free break-even analysis.
CSPR, VVD, bleed rate, steady-state prediction. Compare batch vs fed-batch vs perfusion productivity and media costs. Cell retention device reference.
Answer 6 quick questions about scale, modality, vessel, parameters, environment, and budget. Get ranked sensor recommendations with vendor links covering DO, pH, biomass, CO2, Raman, and NIR.
Find the optimal harvest day from VCD, viability, glucose, and lactate trajectories. Integrated decision rules for CHO mAb, AAV, HEK293, Sf9, and microbial fed-batch.
Plan CAR-T, CAR-NK, iPSC, MSC manufacturing. Calculate expansion requirements, vessel selection, timeline, and cost per dose. Autologous vs allogeneic comparison.
Plan Sf9 / Sf21 baculovirus infections: compute time of infection, MOI, PFU required, infected-cell fraction (Poisson), and harvest window for proteins, VLPs, and AAV.
Calculate multiplicity of infection, virus volume needed, and Poisson infection probabilities. Presets for lentivirus, adenovirus, AAV, baculovirus, retrovirus, vaccinia, and phage.
Generate exponential, linear, and constant feeding profiles from your organism's kinetic parameters. Monod-based predictions for biomass, substrate, and product trajectories.
Scale between vessel sizes using constant kLa, P/V, tip speed, or Re. Calculates impeller RPM, power input, gas flow. Includes standard vessel geometry presets.
Estimate volumetric mass transfer coefficient for stirred tanks (Van't Riet) and shake flasks (Büchs). Compare OTR vs. OUR to predict oxygen limitation.
Calculate OUR, CER and respiratory quotient from inlet/outlet gas compositions using the N₂ balance method. Time-series mode for full fermentation profiles.
Calculate impeller Reynolds number, flow regime, power number (Np), tip speed, P/V and torque. Np vs Re correlation charts. Presets for Rushton, pitched-blade, marine and hydrofoil impellers.
Calculate O2/air/N2/CO2 gas blends for target DO and pH. Sparger flow rates, oxygen enrichment breakpoint analysis, CO2 stripping, gas cost estimation.
Size cooling jackets and coils. Calculate metabolic + agitation heat generation, LMTD, cooling water flow rate. Thermal risk assessment with scale comparison.
Design of Experiments run table generator. Full Factorial, Fractional Factorial, Plackett-Burman, CCD, Box-Behnken, Definitive Screening. Bioprocess presets. CSV export.
Back-of-envelope cost-of-goods per gram of product. Input titer, downstream yield, media cost, run duration. Compare batch vs. fed-batch vs. continuous.
Input your protein properties — get recommended strain, promoter, temperature, IPTG range, induction OD, fusion tags, and chaperone co-expression strategy.
Scan for N-end rule risks, PEST sequences, protease-sensitive sites. Get stabilizing tag recommendations and protease inhibitor cocktail suggestions.
Multi-criteria weighted scoring for clone ranking. Radar chart comparison, traffic light indicators, CSV export. Templates for mAb, biosimilar, bispecific.
Plan MCB/WCB/EoPC campaigns. QC vial allocation, ICH Q5D stability schedule, product lifecycle planning. Calculate vials needed from clinical through commercial.
Model end-to-end mRNA manufacturing: IVT yield, purification waterfall, LNP encapsulation, doses per batch, cost per dose. Scale comparison from 1mL to 5L.
Convert between vg/mL, GC/mL, capsids/mL, and TU/mL with full:empty ratio correction. Dose calculator and doses-per-batch estimator for gene therapy vectors.
Column dimensions, flow rate converter, resin loading, buffer volumes, HETP/plates, and column scale-up. Presets for Protein A, IEX, HIC, SEC, mixed mode.
Column volume (CV), linear ↔ volumetric flow conversion, residence time, column loading capacity, and buffer volume planner. Presets for analytical HPLC, prep HPLC, and bioprocess columns.
Vmax-based sterile filter sizing, disc-to-cartridge scale-up, TFF/UF-DF processing calculator, membrane selection guide. Throughput and flux validation.
Sigma factor method for scaling lab centrifuges to disc stack or tubular bowl. Stokes settling velocity, RPM/RCF converter, Q/Sigma equivalence, separation efficiency.
Track DBC decay over chromatography cycles, predict resin replacement, compare resins (MabSelect, PrismA, Amsphere), CIP impact simulation, cost-per-gram optimization.
Input protein properties (MW, disulfides, pI). Get solubilization buffer, refolding buffer with redox pairs, strategy recommendation, and a print-ready protocol.
Calculate log reduction values per clearance step. Build multi-step clearance strategies. Compare against ICH Q5A regulatory thresholds. Model virus reference panel.
Whether you're growing CHO cells for a monoclonal antibody or fermenting E. coli for a recombinant protein — these tools fit your workflow.
CHO, HEK293, hybridoma for mAbs, vaccines, cell & gene therapy
E. coli, Pichia, yeast expression for enzymes, therapeutics, research reagents
Ethanol, organic acids, amino acids, biopolymers, food & beverage biotech
PhD students, postdocs, and PIs studying microbial physiology, metabolic engineering
Microalgae, cyanobacteria, and plant cell suspension cultures for biofuels, pigments
Clostridium, methanogens, SRB for biogas, syngas fermentation, bioremediation
CAR-T, CAR-NK, iPSC, MSC manufacturing, viral vector production, autologous & allogeneic
Scale-up, tech transfer, process characterization, DoE, PAT implementation
From buffer prep to scale-up. A4, print-ready, free forever. Get updates when new packs drop — no more than monthly.
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