Free tools for fermentation & cell culture scientists
Calculators, trackers, and optimizers built by bioprocess engineers. Replace your scattered Excel sheets with purpose-built tools that work on your phone at the bench. Plus vendor-neutral side-by-side comparisons, independent sensor reviews, and a bioprocess talent pool.
The Toolkit
Every tool a bioprocess scientist needs — from bench to bioreactor. Built for daily use, designed for mobile.
Looking for the method rather than the tool? Read the specific growth rate formula, RPM to RCF conversion, column volume calculation, and primer melting temperature guides.
Lab Essentials
RPM ↔ g-Force (RCF) Converter
Convert RPM to RCF and back instantly. Rotor presets for Eppendorf, Beckman, Thermo Fisher centrifuges. Common protocol quick-reference. Mobile-friendly for bench use.
Protein MW & Extinction Coefficient
Paste amino acid sequence → get molecular weight, extinction coefficient (ε280), A280 for 1 mg/mL, estimated pI, charge vs pH curve. Common protein presets.
Molarity & Solution Calculator
Calculate mass from molarity, percent solutions (w/v, v/v, w/w), concentration unit converter (M to mM, uM, nM). Built-in library of 50+ reagent molecular weights.
Master Mix Calculator
PCR, qPCR, RT-PCR reaction setup for N reactions with dead volume. 6 protocol presets (Standard PCR, SYBR, TaqMan, High-Fidelity, Colony PCR, RT). CSV export and pipetting guide.
OD600 to Cell Density Converter
Convert optical density to cells/mL, CFU/mL, and dry cell weight. Organism presets for E. coli, yeast, Pichia, Bacillus. Dilution factor correction and linearity warnings.
Transfection Calculator
DNA and reagent volumes for PEI (N:P ratio), Lipofectamine 2000/3000, FuGENE HD, CaPO₄. Co-transfection with AAV triple and lentiviral 3rd-gen presets. Scale from 96-well to 50 L bioreactor.
Primer Tm Calculator
Calculate primer melting temperature using nearest-neighbor (SantaLucia 1998), salt-adjusted, and basic methods. Primer pair analysis with quality scorecard, hairpin detection, and adjustable Na+, Mg2+, dNTPs.
DNA / RNA Calculator
A260 to concentration (ng/uL), mass-to-moles conversion (ng/uL to nM/uM for oligos, plasmids, RNA), Nanodrop purity check with 260/280 and 260/230 interpretation.
Buffer Preparation Calculator
Stock solution prep, C1V1=C2V2 dilution, Henderson-Hasselbalch pH buffers, serial dilution planner, and common bioprocess buffer recipes (PBS, Tris, HEPES).
Buffer Recipe Finder
Searchable database of ready-to-weigh bioprocess buffer recipes (PBS, Tris, phosphate, acetate, HEPES, Protein A). Scale masses to any volume with prep steps and a pH selection map.
Cell Counting & Viability Calculator
Hemocytometer cell counter with visual grid input. VCD, viability %, passage planner with vessel and organism presets. Multi-count growth tracking with charts.
Process Monitoring & Analytics
CellTrack — Growth & Viability Tracker
Log VCD, viability, glucose, lactate, titer, pH, DO. Auto-calculates specific growth rate (µ), doubling time, qP, qGlc, IVC. Beautiful charts. CSV export. Works offline.
Golden Batch Analysis & Cross-Run Comparison
Compare bioreactor runs, build golden batch envelopes (mean ± sigma), align batches on biological events, find which variable caused a deviation via z-score contribution. Free SIMCA alternative.
Bioreactor Data Dashboard
Upload CSV from any bioreactor system. Auto-generate time-series charts for DO, pH, VCD, metabolites. Multi-run overlay. Derived metrics (µ, qP, IVC). Data never leaves your browser.
Growth Curve Fitter & µ Calculator
Paste OD or cell count data, auto-fit exponential, logistic, and Gompertz models. Extract µmax, doubling time, lag time. Model comparison with R² and AIC.
Monod Kinetics Calculator
Compute µ from µmax, Ks and substrate S, plot the µ–S curve, and fit µmax & Ks from data (Lineweaver-Burk, Hanes-Woolf, nonlinear). Haldane & dual-substrate models.
Bioreactor Simulator
Watch a live Monod-model fermentation run in batch, fed-batch or chemostat mode — animated vessel, dissolved-oxygen coupling, washout and productivity curves. RK4 in your browser.
ELISA Data Analyzer (4PL Fit)
4-parameter logistic curve fitting for ELISA data. Standard curve with LLOQ/ULOQ, back-calculated sample concentrations, %CV flagging, accuracy validation.
Protein Concentration Calculator
BCA assay, Bradford assay, and direct A280 protein quantification. Standard curve fitting, Beer-Lambert law, dilution correction, and purification yield tracker.
Endotoxin Dilution Calculator
MVD calculation, endotoxin limit by route (IV, intrathecal, ophthalmic), dilution series generator with pipetting volumes, PPC spike recovery validation. USP <85> compliant.
Autoclave F₀ Calculator
Calculate F₀ lethality for autoclave, SIP, and depyrogenation cycles. Moist heat (F₀), dry heat (FH), and depyrogenation (FD) modes with pass/fail validation and interactive charts.
Method Validation Calculator (ICH Q2)
Calculate LOD, LOQ, linearity R², accuracy % recovery, precision %RSD per ICH Q2(R2). Presets for SEC-HPLC, CE-SDS, ELISA, qPCR, BCA, A280, LAL, icIEF. Pass/fail dashboard.
IC50/EC50 Dose-Response Calculator
4-parameter logistic (4PL) curve fitting for dose-response data. Calculate IC50, EC50, IC90, Hill slope, R-squared. Multi-compound comparison. MTT, enzyme, receptor binding, cytotoxicity presets.
Filter Integrity Test Calculator
Bubble point, forward flow, pressure hold and water intrusion specifications for sterile filters. Temperature and altitude corrections. Multi-cartridge scaling. Instant pass/fail with margin analysis.
Clean Room Classification Calculator
Classify cleanrooms per ISO 14644-1 from particle count data. Calculate 95% UCL across sampling locations. Map to EU GMP Grades A-D and FS 209E. At-rest vs in-operation comparison with pass/fail compliance report.
Cleaning Validation (MACO) Calculator
Calculate Maximum Allowable Carryover using dose-based, LD50, 10 ppm, and PDE/HBEL methods. Swab, rinse, and surface residue acceptance criteria. Product pair presets with exportable validation report.
Osmolality Calculator
Estimate media osmolality from component concentrations. Optimal range checker by cell type. Feed impact predictor. Conductivity-to-osmolality correlation.
Cell Culture & Troubleshooting
CHO Cell Culture Troubleshooter
Interactive decision tree for diagnosing common CHO problems. Viability dropping? Low titer? High lactate? Walk through systematic diagnosis with corrective actions.
Seed Train Expansion Planner
Plan your complete seed train from vial to production bioreactor. Auto-selects vessels, calculates timing, media volumes, and generates a visual expansion diagram.
Cell Seeding Calculator
Calculate suspension volume, media volume, split ratio, and total cells needed for any target seeding density. Dual mode: suspension (cells/mL) and adherent (cells/cm²). 15 vessel presets.
Doubling Time Calculator
Calculate cell doubling time, specific growth rate and population doubling level from two cell counts. PDL tracker with passage log. Growth projection mode. CHO, HEK293, Vero, HeLa presets.
HEK293 Growth Database
Published doubling times, growth rates, and culture conditions for HEK293, 293T, 293F, Expi293F, 293-6E, and 293S GnTI⁻ variants. Compare your measured td against reference ranges. Expansion planner from vial to bioreactor.
Microcarrier Planner
Surface area and bead count from carrier load, seeding conversion with the Poisson empty-bead fraction, Zwietering Njs agitation, Kolmogorov shear window and the harvest burst. Cytodex, Hillex II, Synthemax II presets.
Media Formulation & Cost Estimator
Compare basal media costs, calculate supplement concentrations, estimate media cost per liter and per batch. Serum vs serum-free break-even analysis.
Perfusion & Continuous Process Calculator
CSPR, VVD, bleed rate, steady-state prediction. Compare batch vs fed-batch vs perfusion productivity and media costs. Cell retention device reference.
Bioreactor Sensor Selection Tool
Answer 6 quick questions about scale, modality, vessel, parameters, environment, and budget. Get ranked sensor recommendations with vendor links covering DO, pH, biomass, CO2, Raman, and NIR.
Harvest Window Predictor
Find the optimal harvest day from VCD, viability, glucose, and lactate trajectories. Integrated decision rules for CHO mAb, AAV, HEK293, Sf9, and microbial fed-batch.
Cell Therapy Expansion Planner
Plan CAR-T, CAR-NK, iPSC, MSC manufacturing. Calculate expansion requirements, vessel selection, timeline, and cost per dose. Autologous vs allogeneic comparison.
TOI / MOI Optimizer
Plan Sf9 / Sf21 baculovirus infections: compute time of infection, MOI, PFU required, infected-cell fraction (Poisson), and harvest window for proteins, VLPs, and AAV.
MOI Calculator
Calculate multiplicity of infection, virus volume needed, and Poisson infection probabilities. Presets for lentivirus, adenovirus, AAV, baculovirus, retrovirus, vaccinia, and phage.
Process Design & Scale-Up
Fed-Batch Feed Strategy Calculator
Generate exponential, linear, and constant feeding profiles from your organism's kinetic parameters. Monod-based predictions for biomass, substrate, and product trajectories.
Bioreactor Scale-Up / Scale-Down Calculator
Scale between vessel sizes using constant kLa, P/V, tip speed, or Re. Calculates impeller RPM, power input, gas flow. Includes standard vessel geometry presets.
OTR / kLa Estimator
Estimate volumetric mass transfer coefficient for stirred tanks (Van't Riet) and shake flasks (Büchs). Compare OTR vs. OUR to predict oxygen limitation.
kLa Simulator (Gassing-Out)
Run the dynamic gassing-out experiment live. Back-calculate kLa from the DO recovery curve, see how probe lag biases it, and watch OTR meet OUR.
OUR/CER/RQ Off-Gas Analyzer
Calculate OUR, CER and respiratory quotient from inlet/outlet gas compositions using the N₂ balance method. Time-series mode for full fermentation profiles.
Yield Coefficient Calculator
Calculate Yx/s, Yp/s, specific growth rate, qS and qP from fermentation data. Compare your yields to published literature values. Carbon balance closure check.
Reynolds Number Calculator
Calculate impeller Reynolds number, flow regime, power number (Np), tip speed, P/V and torque. Np vs Re correlation charts. Presets for Rushton, pitched-blade, marine and hydrofoil impellers.
Power Number & Torque Calculator
Calculate impeller power number (Np), shaft power, torque and motor sizing. Gassed power correction for aerated bioreactors. Scale-up table at constant P/V. IEC motor recommendation.
Bioreactor Gas Mixing Calculator
Calculate O2/air/N2/CO2 gas blends for target DO and pH. Sparger flow rates, oxygen enrichment breakpoint analysis, CO2 stripping, gas cost estimation.
Bioreactor Heat Transfer Calculator
Size cooling jackets and coils. Calculate metabolic + agitation heat generation, LMTD, cooling water flow rate. Thermal risk assessment with scale comparison.
DOE Experiment Generator
Design of Experiments run table generator. Full Factorial, Fractional Factorial, Plackett-Burman, CCD, Box-Behnken, Definitive Screening. Bioprocess presets. CSV export.
Fermentation Economics Calculator
Back-of-envelope cost-of-goods per gram of product. Input titer, downstream yield, media cost, run duration. Compare batch vs. fed-batch vs. continuous.
Bioprocess LCA Calculator
Screening life cycle assessment for fermentation and cell culture. Get kg CO₂e per kg product, PMI, water and energy intensity, with a hotspot breakdown. Every factor shows its source and confidence tier.
Recombinant Protein Production & Cell Line Development
E. coli Expression Condition Optimizer
Input your protein properties — get recommended strain, promoter, temperature, IPTG range, induction OD, fusion tags, and chaperone co-expression strategy.
Protein Degradation Risk Assessor
Scan for N-end rule risks, PEST sequences, protease-sensitive sites. Get stabilizing tag recommendations and protease inhibitor cocktail suggestions.
Clone Selection Scorecard
Multi-criteria weighted scoring for clone ranking. Radar chart comparison, traffic light indicators, CSV export. Templates for mAb, biosimilar, bispecific.
Cell Bank Sizing Calculator
Plan MCB/WCB/EoPC campaigns. QC vial allocation, ICH Q5D stability schedule, product lifecycle planning. Calculate vials needed from clinical through commercial.
mRNA Process Yield Calculator
Model end-to-end mRNA manufacturing: IVT yield, purification waterfall, LNP encapsulation, doses per batch, cost per dose. Scale comparison from 1mL to 5L.
AAV Titer Unit Converter
Convert between vg/mL, GC/mL, capsids/mL, and TU/mL with full:empty ratio correction. Dose calculator and doses-per-batch estimator for gene therapy vectors.
AAV Production Yield Calculator
Estimate AAV production yields from transfection through purification. Waterfall chart, dose calculator, and cost per dose for AAV2, AAV5, AAV8, AAV9.
VLP Production Yield Calculator
Estimate virus-like particle yields from expression through purification. Waterfall chart, doses per batch, and expression system comparison for Gardasil, Cervarix, Novavax, Hecolin.
Downstream Processing & Purification
Chromatography Simulator
Watch protein bands migrate down the bed and resolve into live peaks. Drag the gradient, flow rate and particle size to trade resolution against pool purity and yield.
Chromatography Column Calculator
Column dimensions, flow rate converter, resin loading, buffer volumes, HETP/plates, and column scale-up. Presets for Protein A, IEX, HIC, SEC, mixed mode.
HPLC Column Volume Calculator
Column volume (CV), linear ↔ volumetric flow conversion, residence time, column loading capacity, and buffer volume planner. Presets for analytical HPLC, prep HPLC, and bioprocess columns.
Filtration & TFF/UF-DF Calculator
Vmax-based sterile filter sizing, disc-to-cartridge scale-up, TFF/UF-DF processing calculator, membrane selection guide. Throughput and flux validation.
Centrifugation Scale-Up Calculator
Sigma factor method for scaling lab centrifuges to disc stack or tubular bowl. Stokes settling velocity, RPM/RCF converter, Q/Sigma equivalence, separation efficiency.
Protein A Resin Lifetime Calculator
Track DBC decay over chromatography cycles, predict resin replacement, compare resins (MabSelect, PrismA, Amsphere), CIP impact simulation, cost-per-gram optimization.
Inclusion Body Refolding Generator
Input protein properties (MW, disulfides, pI). Get solubilization buffer, refolding buffer with redox pairs, strategy recommendation, and a print-ready protocol.
Viral Clearance LRV Calculator
Calculate log reduction values per clearance step. Build multi-step clearance strategies. Compare against ICH Q5A regulatory thresholds. Model virus reference panel.
Endotoxin Clearance LRV Tracker
Track endotoxin clearance across purification steps. Cumulative LRV, final EU/dose, EU/mg. USP <85> pass/fail by route. Process presets for mAb, E. coli, Fc-fusion, mRNA.
HCP Clearance Calculator
Track host cell protein clearance across purification steps. HCP ppm, per-step LRV, cumulative clearance. EMA threshold pass/fail. Process presets for mAb Protein A, Fc-fusion, E. coli, biosimilar.
Built for your bench
Whether you're growing CHO cells for a monoclonal antibody or fermenting E. coli for a recombinant protein — these tools fit your workflow.
Biopharma
CHO, HEK293, hybridoma for mAbs, vaccines, cell & gene therapy
Recombinant Protein
E. coli, Pichia, yeast expression for enzymes, therapeutics, research reagents
Industrial Fermentation
Ethanol, organic acids, amino acids, biopolymers, food & beverage biotech
Academic Research
PhD students, postdocs, and PIs studying microbial physiology, metabolic engineering
Algae & Plant Cell
Microalgae, cyanobacteria, and plant cell suspension cultures for biofuels, pigments
Anaerobic Biotech
Clostridium, methanogens, SRB for biogas, syngas fermentation, bioremediation
Cell & Gene Therapy
CAR-T, CAR-NK, iPSC, MSC manufacturing, viral vector production, autologous & allogeneic
Process Development
Scale-up, tech transfer, process characterization, DoE, PAT implementation
Bioprocess job portal
A job portal for bioprocessing, built on short structured cards instead of CVs. It works both ways. If you are open to a move, put up a card and let matching roles reach you — no CV, no cover letter, no account, and your email address is never published. If you are hiring, filter people by modality, host, discipline, scale and unit operation, which is what you actually screen on and what no general job board lets you search, then message anyone directly through us. Free in both directions.
Upstream process engineer, CHO mAbs
Downstream scientist, AAV and lentivirus
MSAT lead, microbial fermentation
CMC consultant, cell and gene therapy
These four are examples showing the card format, not real people. The pool is new and we are building it from scratch rather than scraping it, so it is small and every card in it is a real person.
Six free PDF packs — pick what you need
From buffer prep to scale-up. A4, print-ready, free forever. Get updates when new packs drop — no more than monthly.
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